rabbit polyclonal anti trpa1 Search Results


anti-trpa1 (extracellular) antibody  (Alomone Labs)
94
Alomone Labs anti-trpa1 (extracellular) antibody
Anti Trpa1 (Extracellular) Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-trpa1 (extracellular) antibody/product/Alomone Labs
Average 94 stars, based on 1 article reviews
anti-trpa1 (extracellular) antibody - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
mouse antibody for trpa1 clone 6g8  (Abnova)
90
Abnova mouse antibody for trpa1 clone 6g8
<t>TRPA1</t> is expressed in atherosclerotic lesions and inhibition of TRPA1 activity with HC030031 exacerbates hyperlipidemia, inflammation and atherosclerosis in apoE -/- mice. Aortas were collected from apolipoprotein E knockout (apoE -/- ) mice and subjected to (A) western blot analysis to evaluate the protein level of TRPA1 and α-tubulin or (B) immunohistochemistry. Immunostaining was with normal rabbit IgG, anti-TRPA1, anti-F4/80 antibody, a macrophage marker, or anti-α-actin, a vascular smooth muscle cell marker. Cell nuclei were stained with hematoxylin. Bar = 50 μm. Four-month-old apoE -/- mice received daily treatment with HC030031 (TRPA1 antagonist, 10 mg/kg body weight) or DMSO (vehicle control) by gastric gavage for 4 weeks. (C and D) Representative sections of aortic roots with H&E staining from each group of mice and quantification of atherosclerotic lesion areas. (E) Quantification of serum levels of total cholesterol, high-density lipoprotein (HDL) cholesterol, non-HDL cholesterol and triglycerides. (F) ELISA of serum levels of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), IL-6, monocyte chemotactic protein 1 (MCP-1) and macrophage inflammatory protein 2 (MIP-2). Data are mean ± SD from 10 mice. *, P < 0.05 vs. WT mice or vehicle treatment.
Mouse Antibody For Trpa1 Clone 6g8, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse antibody for trpa1 clone 6g8/product/Abnova
Average 90 stars, based on 1 article reviews
mouse antibody for trpa1 clone 6g8 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

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TRPA1 is expressed in atherosclerotic lesions and inhibition of TRPA1 activity with HC030031 exacerbates hyperlipidemia, inflammation and atherosclerosis in apoE -/- mice. Aortas were collected from apolipoprotein E knockout (apoE -/- ) mice and subjected to (A) western blot analysis to evaluate the protein level of TRPA1 and α-tubulin or (B) immunohistochemistry. Immunostaining was with normal rabbit IgG, anti-TRPA1, anti-F4/80 antibody, a macrophage marker, or anti-α-actin, a vascular smooth muscle cell marker. Cell nuclei were stained with hematoxylin. Bar = 50 μm. Four-month-old apoE -/- mice received daily treatment with HC030031 (TRPA1 antagonist, 10 mg/kg body weight) or DMSO (vehicle control) by gastric gavage for 4 weeks. (C and D) Representative sections of aortic roots with H&E staining from each group of mice and quantification of atherosclerotic lesion areas. (E) Quantification of serum levels of total cholesterol, high-density lipoprotein (HDL) cholesterol, non-HDL cholesterol and triglycerides. (F) ELISA of serum levels of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), IL-6, monocyte chemotactic protein 1 (MCP-1) and macrophage inflammatory protein 2 (MIP-2). Data are mean ± SD from 10 mice. *, P < 0.05 vs. WT mice or vehicle treatment.

Journal: International Journal of Biological Sciences

Article Title: Transient Receptor Potential Ankyrin 1 Channel Involved in Atherosclerosis and Macrophage-Foam Cell Formation

doi: 10.7150/ijbs.15229

Figure Lengend Snippet: TRPA1 is expressed in atherosclerotic lesions and inhibition of TRPA1 activity with HC030031 exacerbates hyperlipidemia, inflammation and atherosclerosis in apoE -/- mice. Aortas were collected from apolipoprotein E knockout (apoE -/- ) mice and subjected to (A) western blot analysis to evaluate the protein level of TRPA1 and α-tubulin or (B) immunohistochemistry. Immunostaining was with normal rabbit IgG, anti-TRPA1, anti-F4/80 antibody, a macrophage marker, or anti-α-actin, a vascular smooth muscle cell marker. Cell nuclei were stained with hematoxylin. Bar = 50 μm. Four-month-old apoE -/- mice received daily treatment with HC030031 (TRPA1 antagonist, 10 mg/kg body weight) or DMSO (vehicle control) by gastric gavage for 4 weeks. (C and D) Representative sections of aortic roots with H&E staining from each group of mice and quantification of atherosclerotic lesion areas. (E) Quantification of serum levels of total cholesterol, high-density lipoprotein (HDL) cholesterol, non-HDL cholesterol and triglycerides. (F) ELISA of serum levels of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), IL-6, monocyte chemotactic protein 1 (MCP-1) and macrophage inflammatory protein 2 (MIP-2). Data are mean ± SD from 10 mice. *, P < 0.05 vs. WT mice or vehicle treatment.

Article Snippet: Mouse antibody for TRPA1 (clone 6G8, 1:100 for immunohistochemistry or 1:1000 for western blot) was from Abnova (Taoyuan, Taiwan).

Techniques: Inhibition, Activity Assay, Knock-Out, Western Blot, Immunohistochemistry, Immunostaining, Marker, Staining, Control, Enzyme-linked Immunosorbent Assay

Genetic ablation of TRPA1 channel function abolishes the atheroprotective effect of allyl isothiocyanate (AITC) in apoE -/- mice. Four-month-old apoE -/- or apoE -/- TRPA1 -/- mice received daily treatment with AITC (TRPA1 agonist, 10 mg/kg body weight) or DMSO (vehicle control) by gastric gavage for 4 weeks. (A) Immunohistochemistry and quantification of atherosclerotic lesion area in mice. (B-E) Quantification of serum levels of total cholesterol, HDL cholesterol, non-HDL cholesterol and triglycerides. Data are mean ± SD from 10 mice. *, P < 0.05 vs. vehicle-treated apoE -/- mice, # , P < 0.05 vs. AITC-treated apoE -/- mice.

Journal: International Journal of Biological Sciences

Article Title: Transient Receptor Potential Ankyrin 1 Channel Involved in Atherosclerosis and Macrophage-Foam Cell Formation

doi: 10.7150/ijbs.15229

Figure Lengend Snippet: Genetic ablation of TRPA1 channel function abolishes the atheroprotective effect of allyl isothiocyanate (AITC) in apoE -/- mice. Four-month-old apoE -/- or apoE -/- TRPA1 -/- mice received daily treatment with AITC (TRPA1 agonist, 10 mg/kg body weight) or DMSO (vehicle control) by gastric gavage for 4 weeks. (A) Immunohistochemistry and quantification of atherosclerotic lesion area in mice. (B-E) Quantification of serum levels of total cholesterol, HDL cholesterol, non-HDL cholesterol and triglycerides. Data are mean ± SD from 10 mice. *, P < 0.05 vs. vehicle-treated apoE -/- mice, # , P < 0.05 vs. AITC-treated apoE -/- mice.

Article Snippet: Mouse antibody for TRPA1 (clone 6G8, 1:100 for immunohistochemistry or 1:1000 for western blot) was from Abnova (Taoyuan, Taiwan).

Techniques: Control, Immunohistochemistry

Loss of function of TRPA1 channels reverses the anti-inflammatory effect of AITC in apoE -/- mice. ELISA of serum levels of (A) TNF-α, (B) IL-1β, (C) IL-6, (D) MCP-1, and (E) MIP-2. Data are mean ± SD from 10 mice. *, P < 0.05 vs. vehicle-treated apoE -/- mice.

Journal: International Journal of Biological Sciences

Article Title: Transient Receptor Potential Ankyrin 1 Channel Involved in Atherosclerosis and Macrophage-Foam Cell Formation

doi: 10.7150/ijbs.15229

Figure Lengend Snippet: Loss of function of TRPA1 channels reverses the anti-inflammatory effect of AITC in apoE -/- mice. ELISA of serum levels of (A) TNF-α, (B) IL-1β, (C) IL-6, (D) MCP-1, and (E) MIP-2. Data are mean ± SD from 10 mice. *, P < 0.05 vs. vehicle-treated apoE -/- mice.

Article Snippet: Mouse antibody for TRPA1 (clone 6G8, 1:100 for immunohistochemistry or 1:1000 for western blot) was from Abnova (Taoyuan, Taiwan).

Techniques: Enzyme-linked Immunosorbent Assay

Oxidized low-density lipoprotein (oxLDL) triggers TRPA1-dependent Ca 2+ influx in macrophages or TRPA1 re-expressed HEK293 cells. (A) Bone marrow-derived macrophages (BMDMs) were treated with or without oxLDL (50 μg ml -1 ) for the indicated times. (B) BMDMs were pretreated with or without HC010031 (HC, 10 μM) for 1 h, then oxLDL (50 μg/ml) or AITC (10 μM) for an additional 0.5 min. Intracellular Ca 2+ level was measured by Fluo-8 calcium assay kit with green fluorescence detection. (C and D) HEK293 cells were transfected with vector or TRPA1 plasmid for 24 h, then treated with or without oxLDL (50 μg/ml), HC030031 (HC, 10 μM) or AITC (10 μM) for 10 min. Images were photographed by fluorescence microscopy and quantified. Data are mean ± SD from 5 independent experiments. *, P < 0.05 vs. vehicle-treated group, # , P < 0.05 vs. oxLDL-treated group.

Journal: International Journal of Biological Sciences

Article Title: Transient Receptor Potential Ankyrin 1 Channel Involved in Atherosclerosis and Macrophage-Foam Cell Formation

doi: 10.7150/ijbs.15229

Figure Lengend Snippet: Oxidized low-density lipoprotein (oxLDL) triggers TRPA1-dependent Ca 2+ influx in macrophages or TRPA1 re-expressed HEK293 cells. (A) Bone marrow-derived macrophages (BMDMs) were treated with or without oxLDL (50 μg ml -1 ) for the indicated times. (B) BMDMs were pretreated with or without HC010031 (HC, 10 μM) for 1 h, then oxLDL (50 μg/ml) or AITC (10 μM) for an additional 0.5 min. Intracellular Ca 2+ level was measured by Fluo-8 calcium assay kit with green fluorescence detection. (C and D) HEK293 cells were transfected with vector or TRPA1 plasmid for 24 h, then treated with or without oxLDL (50 μg/ml), HC030031 (HC, 10 μM) or AITC (10 μM) for 10 min. Images were photographed by fluorescence microscopy and quantified. Data are mean ± SD from 5 independent experiments. *, P < 0.05 vs. vehicle-treated group, # , P < 0.05 vs. oxLDL-treated group.

Article Snippet: Mouse antibody for TRPA1 (clone 6G8, 1:100 for immunohistochemistry or 1:1000 for western blot) was from Abnova (Taoyuan, Taiwan).

Techniques: Derivative Assay, Calcium Assay, Fluorescence, Transfection, Plasmid Preparation, Microscopy

Ablation of TRPA1 channel function increases oxLDL-induced cholesterol accumulation in macrophages. Wild-type (WT) or TRPA1 -/- BMDMs were pretreated with or without HC030031 (10 μM) or for 1 h, then with 50 μg/ml oxLDL for 24 h. (A and B) Intracellular levels of cholesterol and triglycerides were extracted by use of hexane/isopropanol (3/2, v/v) and analyzed by using colorimetric assay kits. (C) Cells were fixed with 4% paraformaldehyde, then stained with Oil-red O. Cellular nuclei were stained with hematoxylin. Bar = 50 μm. Data are mean ± SD from 5 independent experiments. *, P < 0.05 vs. vehicle-treated group, # , P < 0.05 vs. oxLDL-treated group.

Journal: International Journal of Biological Sciences

Article Title: Transient Receptor Potential Ankyrin 1 Channel Involved in Atherosclerosis and Macrophage-Foam Cell Formation

doi: 10.7150/ijbs.15229

Figure Lengend Snippet: Ablation of TRPA1 channel function increases oxLDL-induced cholesterol accumulation in macrophages. Wild-type (WT) or TRPA1 -/- BMDMs were pretreated with or without HC030031 (10 μM) or for 1 h, then with 50 μg/ml oxLDL for 24 h. (A and B) Intracellular levels of cholesterol and triglycerides were extracted by use of hexane/isopropanol (3/2, v/v) and analyzed by using colorimetric assay kits. (C) Cells were fixed with 4% paraformaldehyde, then stained with Oil-red O. Cellular nuclei were stained with hematoxylin. Bar = 50 μm. Data are mean ± SD from 5 independent experiments. *, P < 0.05 vs. vehicle-treated group, # , P < 0.05 vs. oxLDL-treated group.

Article Snippet: Mouse antibody for TRPA1 (clone 6G8, 1:100 for immunohistochemistry or 1:1000 for western blot) was from Abnova (Taoyuan, Taiwan).

Techniques: Colorimetric Assay, Staining

Inhibition of TRPA1 activity decreases oxLDL-induced cholesterol efflux and upregulation of ABCA1 and ABCG1. (A) For Dil-oxLDL binding assay, WT BMDMs were treated with or without HC030031 (10 μM) for 18 h, followed by 10 μg/ml Dil-oxLDL for an additional 4 h. Cellular lysates were analyzed by fluorometry. (B) Macrophages were treated with HC030031 (10 μM) for 12 h, then NBD-cholesterol (1 μg/ml) for an additional 6 h in the presence of oxLDL, apoAI (10 μg/ml) or HDL (50 μg/ml). Cholesterol efflux was expressed as a percentage of fluorescence in the medium relative to the total amount of fluorescence. Data are mean ± SD from 5 independent experiments. (C) Western blot analysis of SR-BI, ABCA1 and ABCG1 protein levels. Data are mean ± SD from 5 independent experiments. *, P < 0.05 vs. vehicle-treated group, # , P < 0.05 vs.oxLDL-treated group. (D) The capacity of reverse cholesterol efflux of apoE -/- , HC-treated apoE -/- and apoE -/- TRPA1 -/- mice. (E) Western blot analysis and quantitation of protein levels of SR-BI, ABCA1 and ABCG1 in aortas of apoE -/- , HC-treated apoE -/- and apoE -/- TRPA1 -/- mice. Data are mean ± SD from 10 mice. *, P < 0.05 vs. apoE -/- mice.

Journal: International Journal of Biological Sciences

Article Title: Transient Receptor Potential Ankyrin 1 Channel Involved in Atherosclerosis and Macrophage-Foam Cell Formation

doi: 10.7150/ijbs.15229

Figure Lengend Snippet: Inhibition of TRPA1 activity decreases oxLDL-induced cholesterol efflux and upregulation of ABCA1 and ABCG1. (A) For Dil-oxLDL binding assay, WT BMDMs were treated with or without HC030031 (10 μM) for 18 h, followed by 10 μg/ml Dil-oxLDL for an additional 4 h. Cellular lysates were analyzed by fluorometry. (B) Macrophages were treated with HC030031 (10 μM) for 12 h, then NBD-cholesterol (1 μg/ml) for an additional 6 h in the presence of oxLDL, apoAI (10 μg/ml) or HDL (50 μg/ml). Cholesterol efflux was expressed as a percentage of fluorescence in the medium relative to the total amount of fluorescence. Data are mean ± SD from 5 independent experiments. (C) Western blot analysis of SR-BI, ABCA1 and ABCG1 protein levels. Data are mean ± SD from 5 independent experiments. *, P < 0.05 vs. vehicle-treated group, # , P < 0.05 vs.oxLDL-treated group. (D) The capacity of reverse cholesterol efflux of apoE -/- , HC-treated apoE -/- and apoE -/- TRPA1 -/- mice. (E) Western blot analysis and quantitation of protein levels of SR-BI, ABCA1 and ABCG1 in aortas of apoE -/- , HC-treated apoE -/- and apoE -/- TRPA1 -/- mice. Data are mean ± SD from 10 mice. *, P < 0.05 vs. apoE -/- mice.

Article Snippet: Mouse antibody for TRPA1 (clone 6G8, 1:100 for immunohistochemistry or 1:1000 for western blot) was from Abnova (Taoyuan, Taiwan).

Techniques: Inhibition, Activity Assay, Binding Assay, Fluorescence, Western Blot, Quantitation Assay

AITC activation of TRPA1 channels prevents TNF-α-induced inflammation in macrophages. WT BMDMs were pretreated with or without AITC (10 μM) for 1 h, then incubated with TNF-α (10 ng/mL) for an additional 18 h (A-E) or 30 min (F). (A) Griess's assay of levels of nitrite, (B-E) ELISA of IL-1β, MCP-1, IL-6 and MIP-2 in cultured medium and cell lysates. (F) ELISA of cellular NF- k B activity. Data are mean ± SD from 5 independent experiments. *, P < 0.05 vs. vehicle-treated group, # , P < 0.05 vs. TNF-α-treated group.

Journal: International Journal of Biological Sciences

Article Title: Transient Receptor Potential Ankyrin 1 Channel Involved in Atherosclerosis and Macrophage-Foam Cell Formation

doi: 10.7150/ijbs.15229

Figure Lengend Snippet: AITC activation of TRPA1 channels prevents TNF-α-induced inflammation in macrophages. WT BMDMs were pretreated with or without AITC (10 μM) for 1 h, then incubated with TNF-α (10 ng/mL) for an additional 18 h (A-E) or 30 min (F). (A) Griess's assay of levels of nitrite, (B-E) ELISA of IL-1β, MCP-1, IL-6 and MIP-2 in cultured medium and cell lysates. (F) ELISA of cellular NF- k B activity. Data are mean ± SD from 5 independent experiments. *, P < 0.05 vs. vehicle-treated group, # , P < 0.05 vs. TNF-α-treated group.

Article Snippet: Mouse antibody for TRPA1 (clone 6G8, 1:100 for immunohistochemistry or 1:1000 for western blot) was from Abnova (Taoyuan, Taiwan).

Techniques: Activation Assay, Incubation, Enzyme-linked Immunosorbent Assay, Cell Culture, Activity Assay